Kev Npaj Qauv:
Sau cov qauv: Sau cov qauv xws li cov ntshav, ntaub so ntswg, qaub ncaug, thiab zis raws li lub hom phiaj ntawm kev sim.
DNA rho tawm DNA extraction cov khoom siv kom rho tawm DNA los ntawm cov qauv.
PCR cov kev ua haujlwm pib:
Cov Ntawv Cim Tsim: Tsim tshwj xeeb qhia ua haujlwm raws li lub hom phiaj ua ntu zus rau cov DNA ua kom zoo.
Npaj cov tshuaj sib xyaw: suav nrog TEMPLATE DNA, cov ntawv tso cai, DNA polymerase, DNTPS (DUAXYRUCKETIDE), mg 2+, thiab lwm yam.
Teeb PCR program:
PCR yuam kev: Nkag mus rau cov PCR voj voog tsis muaj, xws li 95℃preheating kub, xws li 55℃(xws li 72 degree), thiab tus naj npawb ntawm cov voj voog.
PCR Kev phiv:
Ntxiv cov qauv: ntxiv cov tshuaj tiv thaiv sib tov rau cov pCr kev sib xyaw tank lossis nti, thiab txhua qhov qauv feem ntau muaj ib qho tshuaj tiv thaiv zoo.
Ua PCR: Tsom Xyooj CaCcles raws li Preset program, suav nrog cov cua sov, cua txias, annealing, thiab txuas ntxiv cov kauj ruam.
Tshuaj Ntsuam Xyuas:
Txias tom qab amnification: Tom qab pccr tiav lawm, cov qauv luv luv txias ntawm 4 degree.
Electrophoretic sib cais: Siv Gel Electrophoresis Technology, xws li Agarose Gel Electrophoresis, kom cais cov khoom lag luam ntawm cov khoom sib txawv.
Fluorescence Scanning: Fluorescented sau cov primers emit teeb ci ntawm cov khoom lag luam tshwj xeeb hauv cov khoom lag luam tawm, thiab cov ntsuas pcring cov cim no thiab sau lawv.
Cov ntaub ntawv ua thiab txhais lus:
Cov ntaub ntawv tshawb xyuas software: Siv tshwj xeeb software los tshuaj xyuas cov teeb liab fluorescent thiab xam cov kev xav lossis cov txheeb ze ntawm lub hom phiaj.
Kev txhais lus: Raws li cov ntawv nkhaus thiab lub hauv paus, txiav txim siab seb PCR puas vam meej, puas yog lub hom phiaj tshwm sim, thiab nws ntau npaum li cas.
Qhov tshwm sim kaw thiab khaws cia:
Sau cov txiaj ntsig hauv kev sim daim ntawv qhia thiab khaws cia cov ntaub ntawv thiab cov qauv.