Dengue IGG IGM thiab NS1 Kev Ntsuas sai

Poctell^®Dengue IGG IGM thiab NS1 Cov Ntaus Xov Xwm Ceev Tawm ntawm Dengue Immunoassay IGG thiab tus kab mob antigen (wb ntshav, ntshav ntshav lossis ntshav. Nws yog npaj los siv los ua kev soj ntsuam kuaj thiab raws li kev pabcuam hauv kev kuaj mob nrog tus kab mob Dengue. Tej kev cuam tshuam cov hnoos qeev nrog cov Dengue IGG / iGM / NS1 combo nrawm sim ntaus ntawv yuav tsum tau lees paub nrog lwm txoj kev xeem ntawv thiab nrhiav kev tshawb fawb.

Poctell^®Dengue IGG IGM thiab NS1 Kev Ntsuas sai sai yog qhov tsim nyog cov tshuaj tua kab mob rau cov tshuaj tiv thaiv Dengue hauv tag nrho cov ntshav, ntshav, lossis ntshav. Qhov kev xeem no muaj ob feem, ib qho IGG tivthaiv thiab ib qho IGM tivthaiv. Hauv iGG tivthaiv, tiv thaiv tib neeg IGG yog coated hauv kev xeem kab thaj tsam 1 ntawm kev xeem. Thaum kuaj, qhov hnoos qeev tus mob nrog cov dengue antigen-coated hais nyob rau hauv qhov kev xeem sawb. Cov sib tov ces migrates upward rau ntawm daim nyias nyias muaj nyias thaj chaw 1. Yog tias tus anti-liigand yog coated nyob rau hauv kev xeem kab thaj tsam 2 ntawm kev xeem. Thaum kuaj, tus nqi kho mob me me nrog Ligand los tiv thaiv tib neeg igm. Dengue IGM tshuaj tiv thaiv, yog tias tam sim no hauv cov hnoos qeev, rov qab ntes ntawm cov kab mob antigen-co

Yog li ntawd, yog tias cov hnoos qeev muaj cov tshuaj tiv thaiv kab mob Dengue, ib txoj kab xim av yuav tshwm sim hauv cov kab mob Dengue - yog cov hnoos qeev yuav tshwm sim hauv cov kab xeem, qhia txog qhov tshwm sim tsis zoo. Ua cov txheej txheem kev tswj hwm, kab txheej txheem xim yuav ib txwm hloov pauv hauv cov qauv ntawm cov qauv tswj, qhia tau tias cov hnoos qeev tau ntxiv thiab ua kom muaj qhov tshwm sim.

Poctell^®Dengue NS1 kev ntsuam xyuas sai yog ib sab qab zib ntws chromatographic im impunoassay. Cov kab mob xeem muaj: 1) Cov xim Burgundy contonugate cooj liab (Dengue Ab conjugated Lub t band yog pre-coated nrog luav los tiv thaiv NS1 antigen, thiab C b band yog pre-coated nrog tshis los tiv thaiv tus kab mob igg antibody. Cov tshuaj tiv thaiv rau Denges Anigen lees paub txog kev antigens los ntawm tag nrho plaub lub serotypes ntawm tus kab mob Dengue.

Thaum ib qho kev ntsuas ntawm cov hnoos qeev yog dispensed rau hauv cov qauv zoo ntawm cov kab ke, cov hnoos qeev mig los ntawm cov capillary txiav txim thoob plaws hauv cov kab mob xeem. Dengue NS1 AG yog tias tam sim no hauv cov hnoos qeev yuav khi rau qhov dengue ab conjugates. Lub immunocompluplex yog tom qab ntawd ntes ntawm daim nyias nyias los ntawm cov tshuaj tiv thaiv luav ua ntej, tsim cov xim burgundy y Kev tsis tuaj ntawm t band qhia tias tsis zoo tshwm sim. Qhov kev xeem muaj cov kev tswj hwm sab hauv (C band) uas yuav tsum muaj cov xim burgundy xim ntawm cov immunocomplex ntawm lub xub ntiag ntawm cov xim t band. Txwv tsis pub, qhov kev sim ua yog siv tsis tau thiab qhov hnoos qeev yuav tsum tau rov xeem dua nrog lwm lub cuab yeej.

Cia cov cuab yeej sim, hnoos qeev, tsis tau thiab / lossis tswj kom ncav cuag chav sov (15-30 degree) ua ntej xeem.
1.Thaum 1: Nqa cov quav dev thiab cov khoom sib txuas rau chav sov kom sov yog tias khov. Sib tov cov hnoos qeev zoo ua ntej xav tau ib zaug.
2.Txawm 2: Thaum npaj mus kuaj, Qhib lub hnab ntawm cov ntsiab lus thiab tshem tawm cov cuab yeej. Muab cov cuab yeej kuaj sim ntawm ib qho chaw huv thiab tiaj.
3.Tep 3: Nco ntsoov sau npe rau cov cuab yeej nrog cov hnoos qeev tus lej ID.
4.Tep 4: Sau cov yas depper nrog cov quav. Tuav tus kheej rov qab ntsug, dispense kwv yees li 10 μl piliten. Thiab 2 ~ 3 tee (kwv yees li 80 μl-12L) ntawm buffer rau hauv lub IRG / i poob (kwv yees li ntawm 40μl), nco ntsoov tias tsis muaj pa npuas.
5.Tep 5: Teeb lub sijhawm.
6.Qhov 6: Cov txiaj ntsig tuaj yeem nyeem hauv 15 feeb. Cov txiaj ntsig tau zoo tuaj yeem pom hauv luv li 1 feeb. Tsis txhob nyeem cov txiaj ntsig tom qab 30 feeb.

Cim npe nrov: Dengue IGG IGM thiab NS1 Sai sai, Tuam Tshoj Dengue Dengue IGG igm thiab NS1 Cov Chaw Muag Khoom, Chaw Tsim Tshuaj